• Provides an understanding of which techniques are used in diagnosis at the molecular level


• Explains the basic principles of molecular biology and their application in the clinical diagnosis of diseases


• Places protocols in context with practical applications

Diagnostic Molecular Biology describes the fundamentals of molecular biology in a clear, concise manner to aid in the comprehension of this complex subject. Each technique described in this book is explained within its conceptual framework to enhance understanding.

The targeted approach covers the principles of molecular biology including the basic knowledge of nucleic acids, proteins, and genomes as well as the basic techniques and instrumentations that are often used in the field of molecular biology with detailed procedures and explanations. This book also covers the applications of the principles and techniques currently employed in the clinical laboratory.

Part I
1. Nucleic Acids: DNA and RNA
2. Nucleic Acid-Based Cellular Activities
3. Gene Expression: Transcription of the Genetic Code
4. Gene Expression: Translation of the Genetic Code
5. The Genome

Part II
6. Extraction and Purification of Nucleic Acids and Proteins
7. Detection and Analysis of Nucleic Acids
8. Quantification and Analysis of Proteins
9. Amplification of Nucleic Acids
10. Characterization of Nucleic Acids and Proteins
11. Techniques in Sequencing
12. Genome and Transcriptome Analysis

Part III
13. Molecular Diagnosis of Chromosomal Disorders
14. Molecular Diagnosis of Mutation and Inherited Diseases
15. Molecular Diagnosis of Infectious Diseases
16. Guidance for Molecular Clinical Laboratory